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The Surface Nature of Proteins of a Bovine Enterovirus, Before and After Neutralization

Department of Biochemistry, Queen's University of Belfast, Belfast, Northern Ireland

The surface nature of the proteins of a bovine enterovirus have been determined by using ¹²⁵I and pyridoxal phosphate-sodium borohydride labelling techniques. As found previously, ¹²⁵I labels only VP₁ in intact capsid particles, whereas reaction with pyridoxal phosphate followed by reduction with tritiated sodium borohydride labels VP₁, VP₂ and VP₃. Only VP₄ is found to have no surface tyrosine, histidine or lysine available for reaction. After neutralization with homologous antisera, however, VP₄ becomes exposed and is then available for labelling with ¹²⁵I. This must reflect a substantial conformational change in the virus particle after neutralization.

^* Present address: Medical Research Council Laboratory Animals Centre, Woodmansterne Road, Carshalton, Surrey, SM5 4EF.

Received 17 October 1975; accepted 2 March 1976.

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