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J Gen Virol 32 (1976), 311-314; DOI 10.1099/0022-1317-32-2-311
© 1976 Society for General Microbiology
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The Use of Tris-HCl Buffer for Inoculation of Tomato Protoplasts with Tobacco Mosaic Virus

F. Motoyoshi and N. Oshima

Institute for Plant Virus Research, 959 Aoba-cho, Chiba 280, Japan

When tris-HCL was used as the buffer for inoculation of tomato protoplasts with tobacco mosaic virus, a greater proportion became infected than when phosphate was used. Using 0.05 M-tris-HCl buffer, pH 8.0, in the presence of poly-L-ornithine or poly-D-lysine, 50 to 80% infection was obtained.

Received 26 February 1976; accepted 8 April 1976.




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