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A Rapid Method for the Quantitative Study of RNA from Canine Distemper Virus Infected Cells

Institute for Virology University of Wurzburg West Germany

Centrifugation through CsCl was used to isolate ³²P-labelled RNA in a one-step purification procedure. The method is suitable for quantitative as well as preparative studies and appears to have considerable advantages over conventional methods of RNA extraction. We have used this procedure to investigate the RNA synthesized in Vero cells infected with canine distemper virus (CDV). We show that the combination of CsCl centrifugation and affinity chromatography on poly-U Sepharose provides a rapid method for isolating messenger RNA from virus infected cells.

^* Present address: Department of Biochemistry, Medical Biology Centre, Queen's University, Belfast, Northern Ireland.

Received 19 December 1975; accepted 21 April 1976.