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Centro de Biología Molecular (C.S.I.C.-U.A.M.), Velázquez 144, Madrid-6, Spain
A haemadsorption microtest for African swine fever (ASF) virus is described. This assay is as sensitive and its response is faster than the conventional assay which uses buffy coat cultures in Leighton tubes. The method can also process a larger number of samples by using smaller amounts of swine blood and laboratory space. A plaque assay for ASF virus adapted to grow in VERO cells gives a titre similar to that obtained using the haemadsorption microtest. In both the micromethod and the plaque assay infection may be produced by a single infective particle.
Received 31 December 1975;
accepted 28 April 1976.
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G. Andres, A. Alejo, C. Simon-Mateo, and M. L. Salas African Swine Fever Virus Protease, a New Viral Member of the SUMO-1-specific Protease Family J. Biol. Chem., January 5, 2001; 276(1): 780 - 787. [Abstract] [Full Text] [PDF]
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