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Isolation and Properties of the DNA of African Swine Fever (ASF) Virus

Centro de Biologia Molecular (C.S.I.C.-U.A.M.), Velázquez 144, Madrid-6, Spain

African swine fever (ASF) virus was grown either in swine macrophages or in VERO cells and purified free of cell DNA. Virus DNA was isolated from virions as a molecule with a sedimentation coefficient of 60S and a contour of 58 ± 3 µm. These two values give a mol. wt. of 102 ± 5 x 10⁶ and 107 ± 5 x 10⁶, respectively, for the genome of ASF virus. Denatured DNA fragments from ASF virus reassociate with a C₀t value of 0.60 ± 0.05 Ms, which compared with the corresponding value for T4 DNA gives for the molecular mass of ASF virus DNA a value of 102 ± 8 x 10⁶ daltons. Only virus DNA is synthesized in ASF virus-infected swine macrophages.

Received 31 December 1975; accepted 29 April 1976.

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