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J Gen Virol 33 (1976), 259-266; DOI 10.1099/0022-1317-33-2-259
© 1976 Society for General Microbiology
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Enhancement by Phytohaemagglutinin of Inactivation of Herpes Simplex Virus by Concanavalin A

M. Ito* and A. L. Barron

Department of Microbiology, School of Medicine, State University of New York at Buffalo, Buffalo, New York 14214, and Department of Microbiology and Immunology, College of Medicine, University of Arkansas Medical Sciences Campus, Little Rock, Arkansas 72201, U.S.A.

Inactivation of herpes simplex virus (HSV) by concanavalin A (Con A) was enhanced by treatment with phytohaemagglutinin (PHA)-P using a two-stage reaction procedure. Treatment with PHA alone failed to inactivate HSV. Enhancement of inactivation was also effective when HSV was exposed to PHA first. Our results suggest that the envelope of HSV contains receptor sites for Con A which are important in the infectious process, as well as receptor sites for PHA which are not critical for infectivity. Direct interaction of Con A with PHA was demonstrated and the reaction was reversed by {alpha}-methyl-D-glucoside. The data indicate that PHA stabilized the binding of Con A to the virus since reversal of inactivation by {alpha}-methyl-D-glucoside was minimal following treatment with Con A and PHA. Con A inactivated only enveloped virions and enhancement by PHA was a general phenomenon.

* Present address: Department of Medical Viral Oncology, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, New York 14263, U.S.A.

Received 28 April 1976; accepted 14 July 1976.




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Copyright © 1976 by the Society for General Microbiology.