| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
M.R.C. Virology Unit, Institute of Virology, Church Street, Glasgow, G11 5JR, Scotland
Cells infected with herpes simplex virus type 1 (HSV-1) DNA by the calcium phosphate precipitation technique produce virus which leads to the formation of plaques (Graham, Veldhuisen & Wilkie, 1973). In the study reported here we show that treatment of cell monolayers with dimethyl sulphoxide (DMSO) solutions after infection with DNA-calcium phosphate complexes leads to a considerable increase in the number of plaques obtained. The conditions for this enhancement of infectivity have been optimized for baby hamster kidney (BHK) cells, and increases in plaque numbers of over 100-fold have been obtained. The treatment appears to increase the proportion of cells which respond to DNA infection by initiating plaque formation, and results in a large increase in the measured specific infectivity of HSV-1 DNA. DMSO causes similar (but quantitatively different) responses in various other cell lines infected with HSV-1 DNA. BHK cells infected with either virus particles, or virus DNA by the DEAE-dextran technique (Laithier & Sheldrick, 1975), do not exhibit this massive enhancement following exposure to DMSO.
Received 20 May 1976;
accepted 9 July 1976.
This article has been cited by other articles:
|
|
 |
|
  A.-M. Anesti, P. J. Peeters, I. Royaux, and R. S. Coffin Efficient delivery of RNA Interference to peripheral neurons in vivo using herpes simplex virus Nucleic Acids Res., August 1, 2008; 36(14): e86 - e86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. M. Porter and N. D. Stow Virus particles produced by the herpes simplex virus type 1 alkaline nuclease null mutant ambUL12 contain abnormal genomes J. Gen. Virol., March 1, 2004; 85(3): 583 - 591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Grey, M. Sowa, P. Collins, R. J. Fenton, W. Harris, W. Snowden, S. Efstathiou, and G. Darby Characterization of a neurovirulent aciclovir-resistant variant of herpes simplex virus J. Gen. Virol., June 1, 2003; 84(6): 1403 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ellsmore, G. G. Reid, and N. D. Stow Detection of human cytomegalovirus DNA replication in non-permissive Vero and 293 cells J. Gen. Virol., March 1, 2003; 84(3): 639 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. D. Stow Packaging of Genomic and Amplicon DNA by the Herpes Simplex Virus Type 1 UL25-Null Mutant KUL25NS J. Virol., November 15, 2001; 75(22): 10755 - 10765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Hodge and N. D. Stow Effects of Mutations within the Herpes Simplex Virus Type 1 DNA Encapsidation Signal on Packaging Efficiency J. Virol., October 1, 2001; 75(19): 8977 - 8986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Lilley, F. Groutsi, Z. Han, J. A. Palmer, P. N. Anderson, D. S. Latchman, and R. S. Coffin Multiple Immediate-Early Gene-Deficient Herpes Simplex Virus Vectors Allowing Efficient Gene Delivery to Neurons in Culture and Widespread Gene Delivery to the Central Nervous System In Vivo J. Virol., May 1, 2001; 75(9): 4343 - 4356. [Abstract] [Full Text]
|
|
|
|
|
|
J. L. Arthur, C. G. Scarpini, V. Connor, R. H. Lachmann, A. M. Tolkovsky, and S. Efstathiou Herpes Simplex Virus Type 1 Promoter Activity during Latency Establishment, Maintenance, and Reactivation in Primary Dorsal Root Neurons In Vitro J. Virol., April 15, 2001; 75(8): 3885 - 3895. [Abstract] [Full Text]
|
|
|
|
|
|
A. P. Abbotts, V. G. Preston, M. Hughes, A. H. Patel, and N. D. Stow Interaction of the herpes simplex virus type 1 packaging protein UL15 with full-length and deleted forms of the UL28 protein J. Gen. Virol., December 1, 2000; 81(12): 2999 - 3009. [Abstract] [Full Text]
|
|
|
|
|
|
R. Lachmann, M Sadarangani, H. Atkinson, and S Efstathiou An analysis of herpes simplex virus gene expression during latency establishment and reactivation J. Gen. Virol., May 1, 1999; 80(5): 1271 - 1282. [Abstract]
|
|
|
|
|
|
J. Arthur, R Everett, I Brierley, and S Efstathiou Disruption of the 5' and 3' splice sites flanking the major latency- associated transcripts of herpes simplex virus type 1: evidence for alternate splicing in lytic and latent infections J. Gen. Virol., January 1, 1998; 79(1): 107 - 116. [Abstract]
|
|
|
|
|
|
J. Simas, R. Bowden, V Paige, and S Efstathiou Four tRNA-like sequences and a serpin homologue encoded by murine gammaherpesvirus 68 are dispensable for lytic replication in vitro and latency in vivo J. Gen. Virol., January 1, 1998; 79(1): 149 - 153. [Abstract]
|
|
|
|
 |
|
 M Oskarsson, W. McClements, D. Blair, J. Maizel, and G. Vande Woude Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus Science, March 14, 1980; 207(4436): 1222 - 1224. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
