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J Gen Virol 33 (1976), 517-521; DOI 10.1099/0022-1317-33-3-517
© 1976 Society for General Microbiology

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Purification of Interferon by Adsorption Chromatography on Controlled Pore Glass

V. G. Edy, I. A. Braude, E. De Clercq, A. Billiau and P. De Somer

Rega Institute for Medical Research Katholieke Universiteit Leuven, Minderbroedersstraat 10 B-3000 Leuven, Belgium

Human fibroblast and mouse L929 cell interferons can be purified by adsorption to and subsequent elution from Controlled Pore Glass. Purification of 40 to 90-fold to specific activities of 1 to 5 x 106 units/mg of protein can be achieved in a single step, with good recovery of activity. Human leukocyte interferon does not bind to the glass and cannot be purified in this way.

Received 18 May 1976; accepted 21 July 1976.





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Copyright © 1976 by the Society for General Microbiology.