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Purification of Interferon by Adsorption Chromatography on Controlled Pore Glass

Rega Institute for Medical Research Katholieke Universiteit Leuven, Minderbroedersstraat 10 B-3000 Leuven, Belgium

Human fibroblast and mouse L929 cell interferons can be purified by adsorption to and subsequent elution from Controlled Pore Glass. Purification of 40 to 90-fold to specific activities of 1 to 5 x 10⁶ units/mg of protein can be achieved in a single step, with good recovery of activity. Human leukocyte interferon does not bind to the glass and cannot be purified in this way.

Received 18 May 1976; accepted 21 July 1976.

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