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Properties of Hydrangea Ringspot Virus Ribonucleic Acid

Department of Botany and Plant Pathology, Iowa State University, Ames 50011
^* Department of Plant Pathology, University of Minnesota, St. Paul 55108, U.S.A.

Hydrangea ringspot virus has been purified to near homogeneity. Virus nucleic acid was identified as single-stranded RNA with an S value of 33·6 before and 21·1 after formaldehyde denaturation. Nucleic acid mol. wt. determinations made by linear log sucrose density gradient centrifugation gave values of 2·25 x 10⁶ and 2·14 x 10⁶, respectively, for native and formaldehyde-treated RNA. Electrophoresis on polyacrylamide gels before and after formaldehyde denaturation gave mol. wt. values of 2·64 x 10⁶ and 2·03 x 10⁶. The values from formaldehyde denatured RNAs are considered the most reliable. Thermal denaturation profiles showed a T_m of 55·2 °C in 0·1 M-sodium phosphate, pH 7·0, and a hyperchromicity of 22·4%.

Received 18 May 1976; accepted 4 August 1976.