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J Gen Virol 34 (1977), 177-182; DOI 10.1099/0022-1317-34-1-177
© 1977 Society for General Microbiology
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Aminoacylation of Encephalomyocarditis Virus RNA

I. J. D. Lindley and N. Stebbing

Anti-Virals Department Searle Research Laboratories, High Wycombe, Bucks, U.K.

RNA extracted from purified encephalomyocarditis (EMC) virus (EMC-RNA) can be aminoacylated with synthetase preparations from Escherichia coli, beef and rabbit liver. The extent of aminoacylation is between 0·024 and 0·080 moles per mole EMC-RNA and occurs only with serine. Neither removal of possible low mol. wt. contaminants with 3 M-sodium acetate nor periodate oxidation of the virus RNA affects its aminoacylation capacity.

Received 24 June 1976; accepted 8 August 1976.




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Copyright © 1977 by the Society for General Microbiology.