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Biochemical Characterization of Infantile Gastroenteritis Virus (IGV)

Virology Division, Bureau of Laboratories, Center for Disease Control, Public Health Service, U.S. Department of Health, Education and Welfare, Atlanta, Georgia 30333, U.S.A.

Enzymic and biophysical studies with purified infantile gastroenteritis virus (IGV) nucleic acid indicated that the virion contained a double-stranded RNA genome of approx. 14 x 10⁶ daltons which could be separated by gel electrophoresis into eight bands of RNA which were comprised of 15 RNA species. Two major virus proteins, VP2 (mol. wt. = 135000) and VP8 (mol. wt. = 40000), which composed about 85% of the total virion protein, were detected in IGV particles by polyacrylamide gel electrophoresis. Eight additional minor proteins were also resolved.

Received 25 August 1976; accepted 29 September 1976.