J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 35 (1977), 149-158; DOI 10.1099/0022-1317-35-1-149
© 1977 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cavanagh, D.
Right arrow Articles by Brown, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cavanagh, D.
Right arrow Articles by Brown, F.
Agricola
Right arrow Articles by Cavanagh, D.
Right arrow Articles by Brown, F.

Immunogenic and Cell Attachment Sites of FMDV: Further Evidence for their Location in a Single Capsid Polypeptide

D. Cavanagh*, D. V. Sangar, D. J. Rowlands and F. Brown

Animal Virus Research Institute, Pirbright, Surrey, England

Chymotrypsin cleaves only one of the four major polypeptides of foot-and-mouth disease virus (FMDV serotype O) in situ. This polypeptide (VP1, mol. wt. 29 x 103) was first cleaved into fragments of mol. wt. 20 and 9 x 103 and further cleavage could be prevented by the addition of a large excess of bovine serum albumin. The infectivity of the virus particles at this stage was the same as that of the intact virus although the rate of attachment to BHK 21 cells was slower and the immunogenic activity was reduced. If hydrolysis was allowed to continue, VP1 was cleaved into fragments with mol. wt. r8 and < 9 x 103, similar to those obtained with trypsin, and the virus particles then had a greatly reduced infectivity and a lower immunogenicity. Treatment of strains from five other serotypes of the virus with the two enzymes cleaved only VP1 in each instance and there was a corresponding loss of infectivity. The results are discussed in relation to the location and biological activity of the virus polypeptides.

* Present address: Department of Immunology, Middlesex Hospital Medical School, London.

Received 25 October 1976; accepted 24 November 1976.


This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
M. J. Grubman and B. Baxt
Foot-and-Mouth Disease
Clin. Microbiol. Rev., April 1, 2004; 17(2): 465 - 493.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1977 by the Society for General Microbiology.