HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Frost, E. H. E. Search for Related Content PubMed PubMed Citation Articles by Frost, E. H. E. Agricola Articles by Frost, E. H. E.

Radioactive Labelling of Viruses: an Iodination Technique Preserving Biological Properties

Département de Microbiologie Centre Hospitalier Universitaire Université de Sherbrooke, Sherbrooke, Quebec Canada J1H 5N4

An iodination procedure suitable for the radioactive labelling of viruses to be used in biological experiments is described. It is characterized by the addition of carrier protein to small amounts of virus before iodination with chloramine T, the use of low concentrations of chemicals, and a rapid purification of the labelled virus to minimize radiation inactivation. Using this procedure, polyoma virus was labelled to a specific activity 100 times greater than that which can be obtained with tritiated amino acids, while its sedimentation coefficient, buoyant density, decapsidation and haemagglutinating activity remained unaffected. Reduction in infectivity, possibly due to radiation inactivation, was slight. Similar results were obtained with adenovirus.

^* Present address: Medical Research Council, Experimental Virus Research Unit, Institute of Virology, Church St, Glasgow G11 5JR, Scotland.

Received 25 September 1975; accepted 19 November 1976.

This article has been cited by other articles:

				W. A. Banks, E. O. Freed, K. M. Wolf, S. M. Robinson, M. Franko, and V. B. Kumar Transport of Human Immunodeficiency Virus Type 1 Pseudoviruses across the Blood-Brain Barrier: Role of Envelope Proteins and Adsorptive Endocytosis J. Virol., May 15, 2001; 75(10): 4681 - 4691. [Abstract] [Full Text]