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Production of RNA and Artificial Top Component from Parsley Carrot-leaf Virus Heated In vitro

Istituto di Patologia Vegetale, Università degli Studi, Bari, Italy

The extinction-temperature profile of parsley carrot-leaf virus in 0.02 M-(Na-K) phosphate buffer, pH 7.2, containing 0.1 M-NaCl, was determined. At the T_d (dissociation temperature = 65 °C), the point at which E₂₆₀ begins to increase, the virus particles apparently dissociate to form RNA and empty protein shells (top component). At the T_f (temperature at the inflexion point of the curve = 70 °C), corresponding to half the maximum increase in E₂₆₀, the protein denatures and precipitates. Temperatures below T_d have little effect on the virus, whereas temperatures higher than T_f also degrade the RNA. The results of the present work seem to suggest that the increase in E₂₆₀ of PCLV preparations upon heating is mostly attributable to turbidity caused by coat protein denaturation rather than to effects on RNA.

Received 9 August 1976; accepted 20 October 1976.