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Studies of L Cells Persistently Infected with VSV: Factors Involved in the Regulation of Persistent Infection

Germfree Life Research Institute, Nagoya University School of Medicine, Schowa-ku, Nagoya, Japan

Infection of interferon-treated L cells with VSV led frequently to the establishment of L cells persistently infected with VSV (L_vsv cells). These cells were characterized by the following properties; (1) no supplement of antiviral factors such as anti-VSV antiserum, interferon, was required for their maintenance; (2) virus antigens were detected in about 5 to 30% of the cells by immunofluorescence staining; (3) the cells were not only resistant to superinfection by homologous virus, but also resistant to challenge by heterologous viruses such as Mengo virus; (4) the cells were destroyed by co-cultivation with heterologous cells susceptible to VSV infection; (5) the cells could be cured by serial cultivation in medium containing antiviral antibody, and the cured cells were as susceptible to VSV as normal L cells.

It was shown that at least three factors (interferon, defective interfering [DI] particles and a selection of small-plaque temperature-sensitive [ts] mutants) took part in the maintenance of L_vsv cells although it was difficult to evaluate exactly the relative importance of these factors. The effect of antiviral antibody, interferon and incubation temperature upon the maintenance of L_vsv cells are discussed further.

Received 30 September 1976; accepted 6 December 1976.

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