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J Gen Virol 35 (1977), 335-339; DOI 10.1099/0022-1317-35-2-335
© 1977 Society for General Microbiology

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Cytopathic Effect and Plaque Formation by Arboviruses in a Continuous Cell Line (XTC-2) from the Toad Xenopus laevis

C. J. Leake, M. G. R. Varma and Mary Pudney

Department of Entomology, London School of Hygiene and Tropical Medicine, London WC1E 7HT

Forty-six arboviruses were tested for c.p.e. and/or plaque formation in an amphibian cell line. C.p.e. was observed with a high proportion of the viruses tested. Comparative plaque assay, in the XTC-2 cells at 28 °C and Vero cells at 37 °C, suggests that these systems are comparable in sensitivity and susceptibility to infection. Practical uses of this cell line are discussed.

Received 23 September 1976; accepted 15 December 1976.


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