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End-point Dilution and Plaque Assay Methods for Titration of Cricket Paralysis Virus in Cultured Drosophila Cells

Entomology Division Department of Scientific and Industrial Research Auckland New Zealand

Cricket paralysis virus, an insect picorna-like virus, caused a distinct c.p.e. in cultured Drosophila melanogaster cells, allowing the development of titration methods based on end-point dilution or plaque assay methods. The end-point dilution (TCD₅₀) method is more sensitive and economical than plaque assays and is easily scored. The data indicate a minimum infectivity/particle ratio of about 1/2000.

Received 30 December 1976; accepted 12 January 1977.

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