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J Gen Virol 36 (1977), 123-135; DOI 10.1099/0022-1317-36-1-123
© 1977 Society for General Microbiology
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Simian Virus 40 — Chinese Hamster Kidney Cell Interaction. III. Characteristics of Chemical Induction in a Clone of Virogenic Transformed Cells

A. G. Morris*, Ch. Lavialle, H. G. Suarez, J. Stevenet, S. Estrade and R. Cassingena

Institut de Recherches Scientifiques sur le Cancer, B.P. No. 8, 94800 Villejuif, France

A number of chemical and physical agents were screened to determine their effectiveness in inducing simian virus (SV40) production in a virogenic clone of SV40-transformed Chinese hamster cells. Mitomycin C (MC) was the most effective inducing agent, and MC induction was further characterized. It was found that levels of infectious SV40 DNA were increased above control levels as early as 6 h after addition of MC to the culture medium and reached maximum levels by 48 h. Virus capsid (V) antigen and virions followed with a lag of about 24 h. V antigen production was sensitive to hydroxyurea, suggesting a dependence on virus DNA synthesis. The proportion of virus-producing cells (infectious centres) and the virus burst per cell were both stimulated by MC. Studies of 3H-thymidine incorporation demonstrated that the rate of SV40 DNA synthesis was maximal at 48 h post-induction, at which time cellular DNA synthesis was almost abolished. Caffeine, at doses not toxic to non-induced cells, strongly inhibited SV40 production in both non-induced and induced cells, suggesting some role for DNA repair mechanisms.

* Present address: Department of Biological Sciences, University of Warwick, Coventry, Warwickshire CV4 7AL, U.K.

Received 30 November 1976; accepted 22 February 1977.




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Copyright © 1977 by the Society for General Microbiology.