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Fine Structure of the Iridescent Virus Type I Capsid

Laboratory of Instrumental Methods of Analysis, Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR, Kiev 252627, USSR

The structure of the iridescent virus type I was examined using negative staining technique. After storage for 1 to 2 months at 4 °C or treatment with chloroform, the icosahedral capsid breaks up into its structural elements — pentagons and triangles, each consisting of 31 and 55 subunits respectively. When such a preparation is centrifuged through a linear sucrose gradient (10 to 40%), 5 zones are revealed. An electron microscopic analysis showed the following distribution of the material (from top to bottom of the tube): (1) free triangles; (2) associated triangles, ‘core’; (3) partially disrupted virions; (4) free intact virus particles; (5) virus aggregates.

A model for iridescent virus type I capsid is proposed, consisting of 12 pentagons (372 subunits) and 20 triangles (1100 subunits). The total number of subunits is 1472.

Received 2 October 1976; accepted 11 February 1977.