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A Plaque Assay for Nuclear Polyhedrosis Viruses using a Solid Overlay

Department of Microbiology and Immunology Queen's University, Kingston, Ontario, Canada K7L 3N6

The nuclear polyhedrosis viruses of Trichoplusia ni and Autographa californica produce plaques in monolayers of Spodoptera frugiperda cells under a solid overlay containing agarose as the solidifying agent. Plaques are visible macroscopically after staining the cells with neutral red or with the tetrazolium salt, INT, and a linear dose response is observed. The sensitivity of the assay is less than that obtained using an end-point dilution technique; however, plaque formation does provide a simple means of cloning virus.

Received 28 February 1977; accepted 13 April 1977.