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Ribonucleic Acid Polymerase Activity Associated with Purified Calf Rotavirus

Station de Recherches de Virologie et d'Immunologie (I.N.R.A.), Route de Thiverval, 78850 — Thiverval-Grignon, France

The presence of an RNA-dependent RNA polymerase is demonstrated in purified rotavirus particles. Optimum polymerase activity was found between 45 to 50 °C, at pH 8, and in the presence of 10 mM-magnesium ions. The polymerase product was highly sensitive to pancreatic RNase (97%) in low or high salt concentration. The enzyme was activated by EDTA treatment of intact particles or heat shock. The similarities between reovirus, blue-tongue virus and rotavirus polymerases are discussed.

Received 20 January 1977; accepted 7 April 1977.

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