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Internal Proteins of Influenza Virus: ³⁵S-Methionine Peptide Maps as Genetic Markers

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL

Methods are described for the preparation in vivo of ³⁵S-methionine-labelled influenza viruses, the purification of the nucleoprotein (NP) and matrix (M) proteins and the separation of peptides obtained by protease digestion by two-dimensional thin-layer chromatography. The maps of the M proteins of A/Okuda/57 (H₂N₂) and A/Finland/4/74 (H₃N₂) were very similar overall but differed in three peptides. Hence they could be clearly distinguished. Maps of the NP proteins of the same strains showed a greater number of differences.

A recombinant strain having the haemagglutinin and neuraminidase of the A/Finland/4/74 parent and the virulence of the A/Okuda/57 parent was shown to have the M and P proteins of A/Okuda/57.

Received 10 January 1977; accepted 25 April 1977.