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Physicochemical Properties of a Virulent Lactobacillus Phage Containing DNA with Cohesive Ends

Yakult Institute for Microbiological Research, 1796 Yaho, Kunitachi Tokyo, Japan 186

Physicochemical properties of a virulent Lactobacillus phage, J1, and its DNA were studied. Phage J1 was shown to consist of a hexagonal head 55 nm in diameter and a flexible, non-contractile tail 290 nm in length and 10 nm in width. The buoyant density of the phage in CsCl was measured as 1·49 g/ml. It was found that J1 DNA was a linear duplex molecule with cohesive ends and had a contour length of 12·2 µm. The single strands were intact for the length of the molecule. The melting temperature, T_m, and the buoyant density of J1 DNA correspond to a G + C content of 48% and 45%, respectively.

Received 31 March 1977; accepted 10 June 1977.