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Production of Friend Leukaemia Antigens in Chronically-Infected Cells Treated with Interferon

G. B. Rossi^*,,

Antonina Dole

Laboratory of Cell Biology and Immunology
^* Section of Virology, Laboratory of Bacterial and Viral Diseases, Istituto Superiore di Sanità, Rome
the Chair of General Pathology, University of Camerino, Camerino
Institute of Virology, University of Rome, Rome, Italy

The effect of mouse interferon (ITF) on the expression of Friend leukaemia virus (FLV) and on dimethyl sulphoxide (DMSO)-stimulated haemoglobin synthesis in Friend erythroleukaemic cells (FLC) was studied. Immunofluorescent staining was used to detect intracellular antigens, and incorporation of ³H-uridine into virions to detect extracellular virus release. Interferon markedly inhibited haemoglobin synthesis and FLV production, but enhanced accumulation of virus antigens in the cytoplasm; on the cell surface, however, FLV antigens were present to the same extent whether ITF was present or not. When ITF was removed, virus production rose and intracellular virus antigens fell to the levels of untreated controls.

Received 30 December 1976; accepted 8 June 1977.