HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Saraste, J. Articles by Keränen, S. Search for Related Content PubMed PubMed Citation Articles by Saraste, J. Articles by Keränen, S. Agricola Articles by Saraste, J. Articles by Keränen, S.

RNA Synthesis Directed by a Temperature-Sensitive Mutant of Semliki Forest Virus

Department of Virology, University of Helsinki, Haartmaninkatu 3, SF-00290 Helsinki 29, Finland

RNA synthesis induced by a temperature-sensitive RNA negative mutant, ts-4, of Semliki Forest virus was studied both at the permissive (28 °C) and restrictive temperature (39 °C). At 28 °C ts-4 directed RNA synthesis was qualitatively similar to the wild type but overall synthesis remained somewhat lower. Only about 3% of RNA was synthesized compared to the wild type virus when ts-4 was maintained at 39 °C throughout the infection. The sedimenting radioactivity was almost exclusively in 42S RNA. When ts-4 infected cultures were shifted from 28 to 39 °C at 5 h post-infection, the synthesis of 26S RNA was shut off while 42S RNA synthesis continued. The synthesis of 26S RNA started again even in the presence of cycloheximide if the cultures were shifted back to 28 °C. Our results suggest that 26S RNA synthesis is controlled by a virus-specific protein, the function of which is not required for the synthesis of 42S RNA, In ts-4 this protein is denatured at 39 °C and becomes non-functional, but renatures and becomes functional upon shift down to 28 °C.

Received 3 May 1977; accepted 17 June 1977.

This article has been cited by other articles:

				J Suopanki, D. Sawicki, S. Sawicki, and L Kaariainen Regulation of alphavirus 26S mRNA transcription by replicase component nsP2 J. Gen. Virol., February 1, 1998; 79(2): 309 - 319. [Abstract]