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J Gen Virol 38 (1978), 175-178; DOI 10.1099/0022-1317-38-1-175
© 1978 Society for General Microbiology
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The Replication and Titration of Iridescent Virus Type 22 in Spodoptera frugiperda Cells

D. A. Brown, Thelma Lescott, K. A. Harrap and D. C. Kelly

N.E.R.C., Unit of Invertebrate Virology, 5, South Parks Road Oxford, OX1 3UB U.K.

A plaque assay for iridescent virus type 22 (from Simulium sp.) using Spodoptera frugiperda cells has been devised, and the kinetics of growth of the virus in this cell line have been determined. The virus particle/p.f.u. ratio was 75 ± 8, and the p.f.u./TCID50 ratio was 0.56 ± 0.11.

Received 28 June 1977; accepted 9 August 1977.




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Copyright © 1978 by the Society for General Microbiology.