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J Gen Virol 38 (1978), 209-221; DOI 10.1099/0022-1317-38-2-209
© 1978 Society for General Microbiology
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Properties of a Transforming Virus, KiMSV(RHHV), Isolated from a Co-culture of Rat HTC-H1 Cells with K-NRK Cells

Stringner S. Yang, Harry L. Malech, Roy S. Wu* and Daniel I. Woronow{dagger}

Laboratory of Cell Biology, National Cancer Institute, Bethesda, Md. 20014
* Biotech Research Laboratory, Rockville, Md. 20852, U.S.A.

Some morphological, biological, immunological and biochemical characterizations of a virus, rat helper virus pseudotype Kirsten sarcoma virus, KiMSV-(RHHV), have been presented here. KiMSV(RHHV) has a type C virus ultrastructure. It is strictly rat tropic and is able to transform rat cells in vitro. The possibility of its being a xenotropic mouse virus has been carefully ruled out by exhaustive analyses of host range and immunological studies. Antigenically KiMSV (RHHV) demonstrates cross reactivity with an antiserum specific against rat leukaemia virus, no cross reactivity with antiserum against Moloney leukaemia virus, and only minor cross reactivity with antiserum against cat leukaemia virus. Analysis of virus proteins and glycoproteins by equilibrium acrylamide gradient gel electrophoresis showed that the virus complex possesses both a gp70 fraction and a p30 fraction. KiMSV(RHHV) sediments isopycnically in a linear sucrose gradient at 1.145 to 1.155 g/ml and possesses RNA and reverse transcriptase activity.

{dagger} Present address: Johns Hopkins University, School of Medicine, Baltimore, Md. 21205, U.S.A.

Received 23 March 1977; accepted 9 August 1977.




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Copyright © 1978 by the Society for General Microbiology.