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Suppression of Murine Leukaemia Virus Production by Ouabain and Interferon in Mouse Cells

Department of Microbiology, School of Medicine, Chiba University, Chiba 280, Japan

Ouabain markedly inhibited the growth of the mouse cell lines K3b and JLS-V9 and the production of murine leukaemia virus (MuLV) in them. The inhibition of MuLV production was abolished by exposing the cells to normal medium or by adding a high concentration (43 mM) of K⁺ ions to the ouabain-containing medium. MuLV production was reduced by ouabain more rapidly than host cell directed protein synthesis. After treatment of cells with ouabain (0.5 mM) for 7 h, extracellular reverse transcriptase activities were reduced by 87 to 92%. However, the intracellular level of polymerase activities remained almost unchanged (77 to 98% relative to the control). Mouse interferon inhibited the production of MuLV in K3b cells and this antiviral action was not blocked by 0.5 mM-ouabain.

^* To whom reprint requests should be sent.

Received 27 May 1977; accepted 9 August 1977.