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Defective Particle Assembly in Wild Type P2 Bacteriophage and its Correction by the lg Mutation

S. Jupp

Microbial Genetics Laboratory, Karolinska Institutet, 104 01 Stockholm, Sweden

The mutation lg of phage P2 has been located on the genetic map of P2 to the right of, and closely linked to, the del2 deletion, probably within tail gene F. The lg mutation causes larger burst sizes, compared with the wild type, especially at high incubation temperatures. The frequency of defective particles is lower in preparations of P2 lg than in those of wild type P2. It seems that the mutation lg improves the efficiency of particle assembly.

^* Permanent address: Institute of Microbiology, University of Warsaw, Poland.

Present address: Institute of Molecular Biology, University of Paris, France.

Received 25 April 1977; accepted 15 August 1977.

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