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The Structure of Tobacco Rattle Virus Ribonucleic Acids: Comparison of Large Oligonucleotides Derived from the 3' Ends

G. Darby

A. C. Minson

Department of Virology, The Medical School, University of Birmingham, Birmingham, U.K.

The RNA species of the CAM isolate of tobacco rattle virus have been labelled at their 3' termini and partially digested with T1 ribonuclease. The two RNAs give an identical pattern of labelled fragments following acrylamide gel electrophoresis, showing considerable structural similarity between the 3' ends of these molecules. RNA fragments derived from the 3' ends of short particle RNA and long particle RNA labelled in vitro with ¹²⁵I were tested for their ability to anneal to complementary long particle RNA. Fragments derived from short particle RNA failed to hybridize, showing that despite their structural similarity, the 3' sequences of these RNAs are not homologous and that the homologous sequence known to be shared by TRV RNAs are not at the 3' ends of the molecules.

^* Present address: Station Federale de recherches agronomique, 1260 Nyon, Switzerland.

Present address: Division of Virology, Department of Pathology, University of Cambridge.

Received 4 July 1977; accepted 15 August 1977.