HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Seehafer, J. Articles by Colter, J. S. Search for Related Content PubMed PubMed Citation Articles by Seehafer, J. Articles by Colter, J. S. Agricola Articles by Seehafer, J. Articles by Colter, J. S.

Observations on the Growth and Plaque Assay of BK Virus in Cultured Human and Monkey Cells

Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7

Although human embryo kidney (HEK), muscle (HEM) and lung (HEL) cells are capable of supporting the replication of BK virus (BKV) through passage levels 9, 12 and 12 respectively, only third, fourth and fifth passage level HEK cells were found to be satisfactory for the plaque assay of the virus. BSC-1 and VERO cells can also be used for the plaque assay of BKV. However, in HEK cells plaques can be visualized in 20 days (compared to 28 days in BSC-1 cells), and since in VERO cells the plaques are poorly defined and the titre about 1 log₁₀ lower than in either HEK or BSC-1 cells, HEK cells were the ones chosen for use.

Received 15 August 1977; accepted 20 September 1977.

This article has been cited by other articles:

				P. Randhawa, N. A. Farasati, R. Shapiro, and K. Y. Hostetler Ether Lipid Ester Derivatives of Cidofovir Inhibit Polyomavirus BK Replication In Vitro. Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1564 - 1566. [Abstract] [Full Text] [PDF]