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Mitogen Induction of Murine C-Type Viruses. IV. Effects of Lipoprotein E. coli, Pokeweed Mitogen and Dextran Sulphate

G. Schumann

^* Friedrich Miescher-Institut, CH-4002 Basel
Research Department, Pharmaceutical Division, CIBA-GEIGY Limited, CH-4002 Basel, Switzerland

Lipoprotein E. coli, a B-cell mitogen, is identified as a new agent inducing the release of endogenous C-type virus from mouse spleen cells. Like lipopoly-saccharide, a previously identified inducer, this compound has a synergistic effect with 5-bromo-2'-deoxyuridine. Induced virus has the characteristic density as well as morphology of C-type viruses. Budding viruses are detected on cultured BALB/c cells by electron microscopy 2 to 4 days following culturing in the presence of lipoprotein.

Pokeweed mitogen, a compound mitogenic for T- and B-cells was negative when tested for virus induction, both alone and in combination with 5-bromo-2'-deoxyuridine. Dextran sulphate, another B-cell mitogen, was negative for induction as well. However, when, combined with lipopolysaccharide, it enhanced the virus release induced by this mitogen. In contrast, no additive effects were observed either by combining dextran sulphate with other virus amplifying mitogens or by combinations of mitogens which both have virus-inducing ability. This finding is discussed with respect to B-cell differentiation.

Received 10 May 1977; accepted 29 September 1977.