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6Department of Genetics, University of Helsinki, P. Rautatiekatu 13, SF-00100 Helsinki 10, Finland
Department of Electron Microscopy, University of Helsinki, Malminkatu 20, SF-00100 Helsinki 10, Finland
The freeze-fracturing of the lipid-containing bacteriophage
6 shows the virus membrane to have structural asymmetry with smooth convex and rough concave fracture faces. The host, Pseudomonas phaseolicola, shows the four typical fracture faces of the Gram-negative cell envelope. During the infection of P. phaseolicola by
6 the virus membrane and the bacterial outer membrane fuse to form a bulge. The nucleocapsid obviously also penetrates the cytoplasmic membrane and is seen inside the cell shortly after infection. The outer membrane of the host undulates and after infection small membrane vesicles are formed apparently from the outer leaflet of the outer membrane.
Received 28 July 1977;
accepted 11 November 1977.
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