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Particle Properties and Strains of Andean Potato Mottle Virus

Scottish Horticultural Research Institute, Invergowrie, Dundee, Scotland

Purified preparations of isolate C of andean potato mottle virus (APMV) contained isometric particles c. 28 nm in diam. with sedimentation coefficients (s) of about 53, and 93 and 112S. The two nucleoprotein components, middle (M) and bottom (B), were serologically indistinguishable, and each contained similar relative amounts of two polypeptide species, of mol. wt. 22100 and 41800. M particles had a density in CsCl of 1.41 g/ml, contained a single RNA species of mol. wt. 1.4 x 10⁶ and stained differently from B particles with uranyl formate; B particles had a density of 1.46 g/ml and contained a single RNA species of mol. wt. 2.0 x 10⁶. Preparations of M and B particles were much less infective when inoculated separately than in mixtures. In these properties APMV resembles other comoviruses. The present cryptogram of APMV is R/1:1.4/(27) + 2.0/(34):S/S:S/*, comovirus group.

The two isolates studied, C and H, infected Gomphrena globosa systemically, and therefore differ from the type strain of APMV. Isolate C was antigenically indistinguishable from the type strain and produced similar symptoms in several solanaceous species. Isolate H differed antigenically from C and the type strain, and also differed somewhat in symptomatology and host range.

^* Present address: International Potato Center, Apartado 5969, Lima, Peru.

Received 29 September 1977; accepted 11 November 1977.