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The Multiplication of an Influenza C Virus in an Established Line of Canine Kidney (MDCK) Cells

Department of Virology and Rickettsiology National Institute of Health of Japan 10–35, 2-Chome, Kamiosaki, Shinagawa-ku, Tokyo 141 Japan

JJ/50 and four other strains of influenza C virus grew in an established line of canine kidney (MDCK) cells. Multicycle virus growth was markedly enhanced by the addition of trypsin to the culture medium and these viruses could be passaged serially in this system. The addition of appropriate concentrations of trypsin to the agar overlay medium enabled plaquing of influenza C/JJ/50 virus. Titration by plaque assay on MDCK cells was more sensitive than that by intra-amniotic inoculation of fertile hens' eggs.

Received 28 July 1977; accepted 9 November 1977.