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J Gen Virol 39 (1978), 91-101; DOI 10.1099/0022-1317-39-1-91
© 1978 Society for General Microbiology
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Some Properties of Hepatitis B Core Antigen Isolated from Serum of Infected Humans

A. R. Neurath*, S.-N. Huang{dagger} and N. Strick*

* The Lindsley F. Kimball Research Institute of The New York Blood Center, New York, New York 10021, U.S.A.
{dagger} Pathology Institute, McGill University, Montreal, Quebec H3A284, Canada

The nucleocapsid of Dane particles (= hepatitis B core antigen; HBcAg) was isolated from human sera either positive or negative for e-antigen (HBeAg) — an apparent marker for the level of infectious hepatitis B virus in serum. HBcAg from the HBeAg-positive serum pool consisted of two distinct populations of particles, one with a buoyant density (d) of 1.358 g/ml and a sedimentation coefficient (s20, w) of {approx} 110, and another with d = 1.28 to 1.30 g/ml and s20, w {approx} 70. Only the latter type of particles was isolated from an HBeAg-negative serum pool. HBcAg was labelled with 125I-p-hydroxyphenylpropionic acid N-hydroxysuccinimide ester, dissociated and analysed by polyacrylamide gel electrophoresis. One major and one minor polypeptide with apparent mol. wt. of 16000 ± 500 and 68000, respectively, were detected. Another component having the properties of a glycolipid with a mol. wt. in the order of 103 was observed. After isoelectric focusing, HBcAg was recovered in fractions with a pH between 4.0 and 5.8, suggesting heterogeneity in isoelectric points.

Received 6 September 1977; accepted 27 October 1977.




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Copyright © 1978 by the Society for General Microbiology.