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Physical Maps for HSV Type 2 DNA with Five Restriction Endonucleases

Institute of Virology, M.R.C. Virology Unit, Church Street, Glasgow G11 5JR, Scotland

The ordering of restriction endonuclease fragments of HSV-2 DNA for physical maps has been studied using molecular hybridization techniques and the cleavage of isolated restriction endonuclease fragments with further restriction endonucleases. Physical maps for the fragments produced by EcoRI, Hind III, Bgl II, Xba and Hpa I have been constructed. The mol. wt. of the various regions which constitute HSV-2 genome are very similar to the corresponding mol. wt. in the HSV-1 genome.

Received 1 July 1977; accepted 28 November 1977.

This article has been cited by other articles:

				A. Dolan, F. E. Jamieson, C. Cunningham, B. C. Barnett, and D. J. McGeoch The Genome Sequence of Herpes Simplex Virus Type 2 J. Virol., March 1, 1998; 72(3): 2010 - 2021. [Abstract] [Full Text] [PDF]