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Chromic-Acid Formaldehyde Fixation of Nucleic Acids of Bacteriophage 6 and Infectious Bovine Rhinotracheitis Virus

U.S. Department of Agriculture Agricultural Research Service Department of Plant Pathology University of Nebraska Lincoln, Nebraska, 68583

R. L. Sharpee

Norden Laboratories Inc. Lincoln Nebraska 68528, U.S.A.

Bacteriophage 6 nucleic acid was present as a torus after chromic acid-formaldehyde-OsO₄ fixation and acetone and propylene oxide dehydration. A herpes virus, infectious bovine rhinotracheitis virus, had its DNA mostly as a torus, collapsed in the centre, or as a network, after glutaraldehyde-OsO₄ fixation, but in an uncollapsed torus or network formation after chromic acid-formaldehyde-OsO₄. This fixative stabilized nucleic acids, allowing acetone dehydration and plastic embedding without collapse of nucleic acid to the centre of the virion.

Received 27 September 1977; accepted 7 December 1977.