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J Gen Virol 39 (1978), 449-461; DOI 10.1099/0022-1317-39-3-449
© 1978 Society for General Microbiology
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Electrokinetic Alteration of the Surface of Herpes Simplex Virus Infected Cells

C. J. Thompson and J. J. Docherty*

Department of Microbiology and Cell Biology, S101 Frear Building, The Pennsylvania State University, University Park, PA 16802, U.S.A.

R. C. Boltz, R. A. Gaines and P. Todd

Department of Biochemistry and Biophysics, Althouse Laboratory, The Pennsylvania State University, University Park, Pa 16802, U.S.A.

Changes induced in the ionic composition of the surface of herpes simplex virus (HSV) infected cells were evaluated by whole cell electrophoresis and isoelectric focusing. The kinetics of the progressive decline in cell electrophoretic mobility (a measurement of negative surface charge density) after infection corresponded to the production of infectious virus. The observed HSV-induced increase in cell isoelectric focusing pH demonstrated that the electrokinetic cell surface became ionically more positive. The nature of the ionic changes were investigated using a series of specific reagents in conjunction with cell electrophoresis. Infected cells had 2.4 times more surface binding sites for the amino-specific reagent 4-acetamide-4'-isothiocyanostilbene-2,2'-disulphonic acid. A relationship between these virus-induced amino groups and HSV surface antigens was indicated by the similar kinetics of their appearance and electrophoretic neutralization by HSV antibodies.

* To whom reprint requests should be addressed.

Received 19 August 1977; accepted 7 December 1977.




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Copyright © 1978 by the Society for General Microbiology.