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J Gen Virol 39 (1978), 559-563; DOI 10.1099/0022-1317-39-3-559
© 1978 Society for General Microbiology
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The Structure of the 5'-Terminus of Papaya Mosaic Virus RNA

Mounir AbouHaidar and J. B. Bancroft

Department of Plant Sciences The University of Western Ontario, London, Ontario N6A 5B7, Canada

The RNA of the sinuous papaya mosaic virus particles was labelled in vivo with 32P or in vitro after oxidation by periodate and reduction by KB3H4. After digestion with RNases T1, T2 and A, the oligonucleotides were fractionated by electrophoresis or by chromatography on a DEAE-cellulose column. After such fractionation, an oligonucleotide resistant to the RNases and phosphatase with a structure of m7G5'ppp5'Gp was obtained from the 5'-terminus of the RNA.

Received 16 November 1977; accepted 11 January 1978.




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Copyright © 1978 by the Society for General Microbiology.