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Incorporation of Radioactivity from ¹⁴C-Sugars into Macromolecules in Poliovirus-infected or Guanidine-treated HeLa Cells

Department of Epidemiology and Virus Laboratory, University of Michigan, Ann Arbor, Michigan, U.S.A.

¹⁴Carbon from glucose-U-¹⁴C was found to be incorporated into DNA, RNA and protein in HeLa cell cultures infected with poliovirus. This incorporation was partially inhibited by the 2nd hr of infection. The inhibition was related to the multiplicity of exposure to poliovirus and DNA was inhibited to a greater degree than RNA. In infected and normal HeLa cultures the rate of evolution of ¹⁴CO₂ from glucose-1-¹⁴C was 77 times greater than from glucose-6-¹⁴C. The incorporation of ¹⁴C from these sugars into macromolecules in normal and infected cultures did not reflect this difference, thus the ¹⁴C in the macromolecules comes from the glycolytic pathway rather than the hexosemonophosphate shunt. Concentrations of guanidine inhibiting poliovirus replication also inhibit the incorporation of ¹⁴C from glucose-U-¹⁴C into DNA and RNA in normal and infected cultures. In cultures receiving both virus and guanidine, the inhibitory effects of each were not additive, suggesting that the viral inhibition of ¹⁴C incorporation requires some early step in virus replication.

Received 9 September 1967; accepted 5 July 1968.