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Transformation of Rat Embryo Cells by Adenovirus Type 1

A. M. Lewis, Jun.

I. Macpherson

R. J. Huebner

^* Children's Hospital of Los Angeles, Los Angeles, Calif. 90027.
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Md. 20014,
Institute of Virology, University of Glasgow, Scotland

Primary rat embryo cells were transformed by adenovirus 1. The transformed cells formed foci of multilayered growth in monolayer cultures; cell lines were derived from such foci. A virus stock containing 1.1 x 10⁹ virus particles and 1.5 x 10⁸ p.f.u./ml. contained 2.3 x 10² focus-forming units/ml. when assayed in rat embryo cells. Approximately 4.8 x 10⁶ total virus particles or 6.5 x 10⁵ p.f.u. were required to induce one focus of transformed cells. The transformation rate was 0.0003% for cells exposed to about 2 p.f.u./cell. The transformed cells contained adenovirus 1 specific tumour antigens when tested by immunofluorescent and complement-fixation reactions. They also synthesized adenovirus 1 specific RNA which forms RNase-resistant hybrids with adenovirus 1 DNA.

Received 9 April 1968; accepted 24 June 1968.