HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Watson, D. H. Search for Related Content PubMed PubMed Citation Articles by Watson, D. H. Agricola Articles by Watson, D. H.

The Separation of Herpes Virus-specific Antigens by Polyacrylamide Gel Electrophoresis

Department of Virology, The University of Birmingham, Birmingham 15

Virus-specific antigens in cells infected with herpes simplex virus were separated on a preparative scale by two-stage polyacrylamide-gel electrophoresis. In the first stage, termed ‘electrofiltration’, the extract was electrophoresed through 3.5% gel to remove larger aggregates. Antigens in the resulting filtrate were then separated in the second stage by electrophoresis through 7% gel. The protein was recovered by sectioning the gel followed by overnight elution. Recoveries of about 70% were obtained from a 12 to 15 mg. load. Immunodiffusion and analytical electrophoresis both confirmed that separation had been achieved.

Received 22 May 1968; accepted 9 July 1968.