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The Preparation of ‘Monoprecipitin’ Antisera to Herpes Virus Specific Antigens

Department of Virology, The University of Birmingham, Birmingham, 15

Injection of single precipitin bands (from immunodiffusion tests) into the lymph nodes of rabbits produced antisera reacting in immunodiffusion tests with individual specific antigens of herpes simplex virus. Antisera were made to an antigen obtained by preparative electrophoresis of an extract of cells infected with herpes; neutralization of the infectivity of herpes virus was demonstrated. An antiserum was also prepared to the antigen common to extracts of cells infected with herpes and pseudorabies virus; it contained no neutralizing antibody.

Received 22 May 1968; accepted 9 July 1968.