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The Binding of DNA Polymerase to Polyoma DNA

Medical Research Council Experimental Virus Research Unit Institute of Virology and Institute of Biochemistry University of Glasgow, Scotland

DNA polymerase from unpurified extracts of either infected or serumtreated resting mouse embryo cells was bound to double-stranded polyoma DNA and the complex could be separated from the free enzyme and other protein by zone centrifugation through sucrose gradients. Addition of extract to component I (supercoiled) polyoma DNA converted the DNA to a slower sedimenting form, but had little effect on the sedimentation rate of a mixture of components II and III. DNA extracted from the complex formed from extract and component I had properties indicating that both strands of the DNA were unbroken.

Received 15 May 1968; accepted 25 July 1968.