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Infectivity and Capacity for DNA Replication of Vaccinia Virus Irradiated by -Rays

Groupe de Recherches sur la Pathogénie des Infections Virales de l'I.N.S.E.R.M. Institut de Bacteriologie, 3 rue Koeberlé, Strasbourg, France

Purified vaccinia virus was irradiated by -rays under direct-effect conditions. The ability of the irradiated samples to form plaques (infectivity) and to induce viral DNA synthesis was determined. The radiosensitive volume of the viral unit causing infection (1.9 x 10^-17 cm.³) is very small compared with the volume of the whole viral DNA ( 10%).

The inactivation of the DNA replication function follows a simple exponential law. The radiosensitive volume necessary for the replication of DNA (1.6 x 10^-18 cm.³) represents only 8.5% of the DNA necessary for infectivity and 0.85% of the total viral DNA. This indicates existence of a dissociation between two functions of vaccinia virus, the synthesis of viral DNA and infectivity.

Received 10 June 1968; accepted 1 August 1968.