HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wicker, R. Articles by Avrameas, S. Search for Related Content PubMed PubMed Citation Articles by Wicker, R. Articles by Avrameas, S. Agricola Articles by Wicker, R. Articles by Avrameas, S.

Localization of Virus Antigens by Enzyme-labelled Antibodies

Institut de Recherches Scientifiques sur le Cancer, 94-Villejuif, France

Three enzymes—horseradish peroxidase, alkaline phosphatase, and glucose oxidase—were used as markers in antibody conjugates employed for the detection in the light microscope of either or both T antigens and structural antigens of three viruses: SV40, adenovirus 12 and rat K virus, in infected cell cultures. The specificity of reaction and localization of the antigens was the same with each enzyme and identical with those revealed by fluorochromeconjugates. By using enzymes with reaction products of different colours, two antigens were revealed simultaneously in a single preparation.

Received 13 August 1968; accepted 10 October 1968.