HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shimizu, Y. Articles by Sasahara, J. Search for Related Content PubMed PubMed Citation Articles by Shimizu, Y. Articles by Sasahara, J. Agricola Articles by Shimizu, Y. Articles by Sasahara, J.

Porcine Kidney Cell Line Persistently Contaminated with Avirulent Swine Fever Virus

Swine Fever Research Section National Institute of Animal Health, Kodaira, Tokyo, Japan

The porcine kidney cell line PK-2a has been widely used for studies on Japanese B encephalitis virus, which has a consistently severe cytopathic effect on the cells (Inoue & Ogura, 1962). Whilst attempting to attenuate swine fever virus using this cell line, an avirulent strain of the virus was detected as a contaminant in normal uninoculated cells because it interfered with titration of swine fever virus by the END (exaltation of Newcastle disease virus) method. The END method, which was devised and designated by Kumagai et al. (1961), is an in vitro technique for detecting and titrating non-cytopathic swine fever virus. The method is based on an enhancing effect of swine fever virus on Newcastle disease virus in swine-testicle-cell cultures. The present report described the evidence for the existence of the contaminant virus in PK-2a cells.

The porcine kidney cell lines PK-2a were obtained from four laboratories in Japan.

Received 8 July 1968; accepted 30 December 1968.