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J Gen Virol 40 (1978), 225-228; DOI 10.1099/0022-1317-40-1-225
© 1978 Society for General Microbiology
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A Direct Plaque Assay for Hog Cholera Virus

H. Laude

Station de Recherches de Virologie et d'Immunologie (INRA) Laboratoire de Pathologie Porcine 78850-Thiverval-Grignon, France

Direct plaque formation with representative strains of hog cholera virus (HCV) has been obtained using several pig kidney cell lines under agar overlay. HCV-infected cells appear as hazy plaques when viewed against an indirect light source, and as white plaques after neutral red staining. HCV assay by direct plaque procedure is rapid and convenient and gives infectivity titres identical to the fluorescent focus assay technique.

Received 24 November 1977; accepted 17 January 1978.




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Copyright © 1978 by the Society for General Microbiology.