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J Gen Virol 40 (1978), 229-232; DOI 10.1099/0022-1317-40-1-229
© 1978 Society for General Microbiology
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Effect of Temperature on the Order of Electrophoretic Migration of Influenza Virus Neuraminidase and Nucleoprotein Genes in Acrylamide Gels Lacking Denaturing Agents

Nancy J. Cox and Alan P. Kendal

WHO Collaborating Center for Influenza Virology Division, Center for Disease Control Public Health Service U.S. Department of Health, Education and Welfare Atlanta, Georgia 30333, U.S.A.

When subjected to electrophoresis at 33 °C in 3% polyacrylamide gels with no urea added, the nucleoprotein and neuraminidase genes of an H2N2 and H3N2 virus migrate as RNA bands 5 and 6 respectively. If the temperature of electrophoresis is increased to 46 °C, however, this order of migration is reversed.

Received 7 December 1977; accepted 19 January 1978.




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Copyright © 1978 by the Society for General Microbiology.